Astronauts Identify Mystery Microbes in Space for the 1st Time

Via space.com by Sarah Lewin

NASA astronauts successfully sequenced the DNA of microbes found aboard the International Space Station, marking the first time unknown organisms were sequenced and identified entirely in space. The astronauts found the mystery microbes were two commonly associated with the human microbiome.

Previously, microbes had to be sent to Earth for analysis, and this new sequencing marks an important step in diagnosing astronaut illnesses and, someday, identifying any DNA-based life found on other planets, NASA officials said in a statement. Researchers back on Earth have now verified the microbe identifications are correct, marking the experiment a success.

As a part of the Genes in Space-3 mission, astronauts on the space station last year touched a petri plate to surfaces on the space station and grew the bacteria found there into colonies, which NASA astronaut Peggy Whitson used to amplify and then sequence their DNA. In July 2016, NASA astronaut Kate Rubins became the first to sequence DNA in space, but this latest experiment was both the first time cells were transferred for analysis and the first time unknown organisms were identified in space. (Rubins used mouse DNA sent from Earth.)


As Whitson led the space station experiment, she was guided by NASA microbiologist Sarah Wallace and her team at Johnson Space Center in Houston. But at a critical time, as Whitson prepared to sequence the DNA, Hurricane Harvey intervened.

"We started hearing the reports of Hurricane Harvey the week in between Peggy performing the first part of collecting the sample and gearing up for the actual sequencing," Wallace said in the statement. Ultimately, the Payload Operations Integration Center at NASA's Marshall Space Flight Center in Huntsville, Alabama, helped to connect Whitson and Wallace through Wallace's personal phone, and she guided Whitson to sequence the DNA before sending the data back to Houston.

During analysis, "Right away, we saw one microorganism pop up, and then a second one, and they were things that we find all the time on the space station," Wallace said. "The validation of these results would be when we got the sample back to test on Earth."

Whitson and the samples traveled back to Earth in September 2017, when the next phase of the Genes in Space-3 mission began. Scientists sequenced the microbes again on Earth and verified that each had been identified correctly.

NASA spokesperson Dan Huot told Space.com that of the three colonies grown and then sequenced on the space station, one ended up being Staphylococcus capitis and two were Staphylococcus hominis.
"Staphylococcus hominis and Staphylococcus capitis are commonly associated with the human microbiome," Huot said in an email. "These are very benign microbes and are commonly observed in human-occupied areas and have been identified in samples returned from the ISS previously. Neither of the two species are considered pathogens."

Before this experiment, astronauts had amplified DNA for analysis on the space station using a device called the miniPCR thermal cycler, and they had sequenced a DNA sample with the so-called MinION device. But at last, they had successfully combined the two, NASA officials said.

"It was a natural collaboration to put these two pieces of technology together, because individually, they're both great," Wallace said, "but together, they enable extremely powerful molecular biology applications."

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